About DrugKiNET ...
DrugKiNET is an open-access, online resource developed by Kinexus Bioinformatics Corporation to
foster the identification and characterization of inhibitors of protein kinases for academic and industrial
research. It features comprehensive information on over 800 compounds that have been experimentally
determined to inhibit human protein kinases. This includes the retrieval of the lowest reported
compound IC50, Ki and Kd values from several sources, including the National Center for Biotechnology
Information (NCBI) PubChem Compound database, the Kinase SARfari database from the European
Molecular Biology Laboratory (EMBL) European Bioinformatics Institute, The International Centre
for Kinase Profiling at the University of Dundee, Ambit Biosciences and hundreds of original research
publications. In some cases, estimates for IC50 values were derived from limited measurements of
kinase inhibition at only one to three different concentrations of the compounds. Using over 105,000
experimentally tested, non-redundant kinase-compound pairs for training, we have developed two
kinase inhibitor prediction algorithms to further predict another 200,000 kinase-compound interactions.
Instructions ...
DrugKiNET is designed to be fast and simple to navigate. Just follow the steps outlined in each query.
Presently, there are 4 different types of queries that you can perform with DrugKiNET. You can search
for target protein kinases if you know its UniProt ID, RefSeq ID, or one of its common gene or protein
names. You can also query for a compound of interest if you know its CAS ID, PubChem CID, ChEMBL
ID or one of its common names. A list of possible options for proteins or compounds are
generated by typing at least two letters of their names or identification numbers, and waiting for a few
moments for a complete list to appear. The optimum web browsers for using DrugKiNET are Internet
Explorer, Firefox and Safari.
Other Useful Online Resources From Kinexus ...
Several other useful open-access, online resources are available from Kinexus Bioinformatics
Corporation to foster cell signalling research. Direct links to several of our other knowledgebases
in the SigNET KnowledgeBank are provided just below the header at the top of each webpage in
DrugKiNET. TranscriptoNET provides gene expression information on over 21,000 human genes.
PhosphoNET features data on nearly 1 million confirmed or predicted human phosphorylation sites in
the proteins encoded by these genes. KinATLAS reveals interactions between these proteins with each
other in signalling systems as well as interactions with kinase inhibitory compounds. KinaseNET offers
detailed information about human protein kinases, whereas DrugKiNET has data on over 800 known
compounds that inhibit protein kinases. Additional knowledgebases are planned for human protein
phosphatases, adapter, stress proteins and transcription factors. For semi-quantitative data on actual
protein expression in over 10,000 diverse biological specimens, please also visit our open-access KiNET
DataBank with immunoblotting and antibody microarray analyses with over 800 pan- and phosphosite-
specific antibodies.
Funding, Sponsorship, and Advertising Opportunities ...
To develop and sustain this free resource, we are seeking sponsorship from foundations and
corporations. We gratefully acknowledge financial assistance for DrugKiNET from the National Research
Council of Canada Industrial Assistance Program and the Natural Sciences and Engineering Research
Council of Canada. It is possible for commercial vendors of signal transduction reagents and services to
advertise their relevant products and services on this site. Interested parties should contact our Sales
and Marketing Department at info@kinexus.ca or call toll free in North America 1-866-KINEXUS.
